A Novel Proximity Biotinylation Assay Based on the Self-Associating Split GFP1–10/11
نویسندگان
چکیده
منابع مشابه
Kinase assay based on thiophosphorylation and biotinylation.
Protein kinases catalyze the transfer of the gamma-phosphate group from ATP to a serine, threonine or tyrosine residue of an acceptor protein. These enzymes play an important role in signal transduction. New inhibitors for these enzymes are actively being sought. In this article, we present a novel approach for detecting the activity of protein kinases, which could be useful for the high-throug...
متن کاملBiotinylation by antibody recognition - A novel method for proximity labeling
Identification of protein-protein interactions is a major goal of biological research. Despite technical advances over the last two decades, important but still largely unsolved challenges include the high-throughput detection of interactions directly from primary tissue and the identification of interactors of insoluble proteins that form higher-order structures. We have developed a novel, pro...
متن کاملCharacterization of a novel self-associating Mr 40,000 platelet glycoprotein.
A novel platelet glycoprotein has been purified and characterized. This glycoprotein, designated Pltgp40, is an acidic sialylated 40,000-dalton protein that bears both O-linked and N-linked oligosaccharides. Treatment of Pltgp40 with neuraminidase resulted in a 5,000-dalton reduction in its Mr and a 1.5 Unit alkaline shift in the isoelectric point, indicating the presence of a large number of s...
متن کاملA novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes
Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-co...
متن کاملGeneration of a dual-functional split-reporter protein for monitoring membrane fusion using self-associating split GFP.
Split reporter proteins capable of self-association and reactivation have applications in biomedical research, but designing these proteins, especially the selection of appropriate split points, has been somewhat arbitrary. We describe a new methodology to facilitate generating split proteins using split GFP as a self-association module. We first inserted the entire GFP module at one of several...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proteomes
سال: 2020
ISSN: 2227-7382
DOI: 10.3390/proteomes8040037